I was kind of new in exosap treatment. Always had a kind of secondary bands in the PCR products and had to cut the band from the gel. So after coming here when i first did the exosap treatment, it was fun and i followed the protocol and it worked well. But in my next experiment I vortexed the plates to mix the solutions and all went wrong. I got multiple peaks in the sequences and everything was nothing but a mess. ExoSAP has a less stabilty and u should be gentle with it. So no vortexing next time, just a gentle tapping will do your work fine :)
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