Adding journal to NCBI

http://www.pubmedcentral.nih.gov/about/authorms.html. - More details are in the Technical Bulletin article at http://www.nlm.nih.gov/pubs/techbull/ma01/ma01_locatorplus.html - The difference between PubMed and MEDLINE is explained at http://www.nlm.nih.gov/pubs/factsheets/dif_med_pub.html To identify these journals, go to the indexing information in LocatorPlus. - Start at http://locatorplus.gov/ - Search for the journal record with a Journal Title Search in LocatorPlus - Go to the Details screen If the Indexed In section reads: "Date range of indexed citations unspecified," only selected articles from that journal are in PubMed. The NLM will not add citations from these journals. Information about journal selection is available at: http://www.nlm.nih.gov/pubs/factsheets/j_sel_faq.html

Science in Simple Bangla

I liked these vdo from Dr. Abed chaudhury, will be helpful to make understand the science we do to mass ppl.

http://www.youtube.com/watch?v=urrTsdnf6Vg

http://www.youtube.com/watch?v=nfFmc_E-1L8

http://www.youtube.com/watch?v=3_z3aoYtEAs

Never vortex seuquencing reactions while doing exo sap treatment

I was kind of new in exosap treatment. Always had a kind of secondary bands in the PCR products and had to cut the band from the gel. So after coming here when i first did the exosap treatment, it was fun and i followed the protocol and it worked well. But in my next experiment I vortexed the plates to mix the solutions and all went wrong. I got multiple peaks in the sequences and everything was nothing but a mess. ExoSAP has a less stabilty and u should be gentle with it. So no vortexing next time, just a gentle tapping will do your work fine :)

Reverse Transcriptase PCR dilemma: when to use oligodT and when to Random hexamer

During reverse transcription we can have confusion that when to use oligodT and when to use the Random hexamers. Let us think about how the things work.

The first thing is we use oligodT in cDNA priming when we have the primer in 3 prime end. Cause oligo dT starts annealing from the Poly A site. and if the mRNA is long in size then it cant reach the 5 prime end and drop

Ranom heamers anneal randomly and anneal in any place but it can't annal in extreme 3 prime ends.

So often may labs use a mixture of oligodT and random hexamer to prime cDNA but it is not recommended when synthesize full length cDNA or cDNA library.

Another thing is

"Oligo dT and random primers anneal at low temperature and normally the reaction is recommended to be carried out at ~42°C. However, the use of longer primers such as oligo dT

(20–25) or random decamers, enables the RT reaction to occur at higher temperature (50°C)